Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

3.6K
Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...
3.6K
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

8.1K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
8.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Air pollution-induced airway epithelial barrier dysfunction in COPD: a narrative review of mechanisms and therapeutic implications.

European respiratory review : an official journal of the European Respiratory Society·2026
Same author

Artificial intelligence for scoliosis surgical planning and postoperative prediction.

NPJ digital medicine·2026
Same author

SLC1A5 augmentation bypasses NK cell transduction barriers to deliver complex CAR payloads.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same author

Enriched Environment Suppresses Neuronal Ferroptosis Through SIRT1/AKT/GSK3β-Dependent Glycogen Metabolic Reprogramming After Cerebral Ischemia-Reperfusion.

Antioxidants (Basel, Switzerland)·2026
Same author

Multi-scale generative adversarial network: three-dimensional reconstruction of the scoliotic spine from biplanar X-rays.

Quantitative imaging in medicine and surgery·2026
Same author

Posterior spinal fusion for Lenke 1 C and 2 C AIS: can the lowest instrumented vertebra stop at the lumbar apex?

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2026

Related Experiment Video

Updated: Mar 14, 2026

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography
07:32

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography

Published on: March 30, 2022

5.2K

Matrix metalloproteinases as reagents for cell isolation.

Anna M Knapinska1, Sabrina Amar1, Zhong He2

  • 1Florida Atlantic University, Department of Chemistry & Biochemistry, Jupiter, FL 33458, United States; Torrey Pines Institute for Molecular Studies, Departments of Chemistry and Biology, Port St. Lucie, FL 34987, United States.

Enzyme and Microbial Technology
|October 6, 2016
PubMed
Summary

This study introduces a novel method for isolating adipose-derived stem cells (ADSCs) using matrix metalloproteases (MMPs), improving purity and viability for transplantation. This advancement offers a superior alternative to traditional bacterial collagenase methods.

Keywords:
Adipose tissueClostridium histolyticum collagenaseE. coliMatrix metalloproteinaseRecombinant enzymeStem cell

More Related Videos

A Method of Targeted Cell Isolation via Glass Surface Functionalization
10:40

A Method of Targeted Cell Isolation via Glass Surface Functionalization

Published on: September 20, 2016

10.0K
Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels
07:39

Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels

Published on: January 22, 2019

8.0K

Related Experiment Videos

Last Updated: Mar 14, 2026

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography
07:32

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography

Published on: March 30, 2022

5.2K
A Method of Targeted Cell Isolation via Glass Surface Functionalization
10:40

A Method of Targeted Cell Isolation via Glass Surface Functionalization

Published on: September 20, 2016

10.0K
Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels
07:39

Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels

Published on: January 22, 2019

8.0K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Regenerative Medicine

Background:

  • Traditional cell isolation methods for therapeutic purposes, like stem cell isolation using bacterial collagenases, have remained largely unchanged since the 1980s.
  • Bacterial collagenases are prone to autodegradation and can introduce endotoxin contamination, negatively impacting cell viability and downstream therapeutic applications.
  • Existing methods may lead to reduced efficacy and potential toxicity in cell-based therapies.

Purpose of the Study:

  • To introduce and evaluate a novel method for isolating adipose-derived stem cells (ADSCs) using recombinantly produced matrix metalloproteases (MMPs).
  • To compare the efficacy and purity of ADSCs isolated by MMPs against traditional methods using bacterial collagenase I and Liberase™.
  • To assess the potential of MMP-isolated ADSCs for therapeutic applications, focusing on cell characteristics and differentiation potential.

Main Methods:

  • Isolation of ADSCs from adipose tissue utilizing recombinantly produced matrix metalloproteases (MMPs).
  • Comparative analysis of ADSCs isolated by MMPs, bacterial collagenase I, and Liberase™.
  • Assessment of morphological and phenotypical characteristics, including surface marker expression (CD73, CD90, CD105) via PE staining.
  • Evaluation of adipogenic and osteogenic differentiation potential of isolated ADSCs.

Main Results:

  • ADSCs isolated by MMPs exhibited morphological and phenotypical characteristics comparable to those isolated by collagenase I and Liberase™.
  • Surface marker expression (CD73, CD90, CD105) was similar between MMP- and Liberase™-isolated ADSCs.
  • MMP-isolated ADSCs demonstrated retained adipogenic and osteogenic potential.
  • ADSCs isolated by Liberase™ showed 6% contamination, while MMP-treated samples had <1% contamination, indicating superior purity.

Conclusions:

  • Recombinant MMPs offer a novel and effective method for isolating highly pure ADSCs from adipose tissue.
  • MMP-based cell isolation significantly reduces contamination compared to existing commercial enzyme preparations like Liberase™.
  • This improved cell isolation technique holds promise for optimizing cell transplantation technologies and enhancing the safety and efficacy of cell-based therapies.