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Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

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l-Ornithine affects peripheral clock gene expression in mice.

Takafumi Fukuda¹, Atsushi Haraguchi², Mari Kuwahara²

¹Research Laboratories for Health Science &Food Technologies, Kirin Company, Ltd., Yokohama, Kanagawa, Japan.

Scientific Reports

|October 6, 2016

Summary

L-ornithine, a non-protein amino acid, influences the body's internal clock. This study shows it can adjust peripheral circadian clock gene expression, potentially impacting health and sleep quality.

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Area of Science:

Chronobiology
Nutritional Science
Molecular Biology

Background:

The peripheral circadian clock synchronizes to environmental cues like feeding times.
Specific food components influencing this clock are not fully understood.
L-ornithine, a non-protein amino acid, is known for physiological benefits, including improved sleep quality.

Purpose of the Study:

To investigate the effect of L-ornithine on peripheral circadian clock gene expression in vivo.
To explore the potential mechanisms by which L-ornithine influences the circadian rhythm.

Main Methods:

In vivo monitoring of PER2 expression rhythm in mice.
Oral administration of L-ornithine during the early inactive period.
Analysis of PER2 expression in mouse embryonic fibroblasts.
Measurement of plasma levels of insulin, glucose, and glucagon-like peptide-1.

Main Results:

Repeated oral L-ornithine administration induced a phase advance in the PER2 expression rhythm in mice.
L-ornithine did not directly affect PER2 expression in mouse embryonic fibroblasts, suggesting an indirect mechanism.
L-ornithine increased plasma insulin, glucose, and glucagon-like peptide-1 levels, correlating with mPer2 expression.

Conclusions:

L-ornithine influences peripheral clock gene expression, likely through insulin secretion.
These findings suggest L-ornithine's potential role in regulating circadian rhythms.
L-ornithine may be considered for its health benefits related to circadian rhythm and sleep quality.