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Updated: Mar 14, 2026
10:51
Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
Published on: November 15, 2013
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Visualizing the Architectures and Interactions of Nuclear Receptors
Sepideh Khorasanizadeh1, Fraydoon Rastinejad1
1Integrative Metabolism Program, SBP Medical Discovery Institute, Orlando, Florida 32827.
Endocrinology
|October 6, 2016
Summary
Nuclear receptors (NRs) control gene expression via ligands and protein interactions. Recent structural and proteomic studies reveal how NRs bind DNA and interact with cofactors within chromatin.
Area of Science:
- Molecular Biology
- Genetics
- Structural Biology
Background:
- Nuclear receptors (NRs) are crucial regulators of genetic programs in animals.
- Ligands binding to NRs modulate their interactions with transcriptional coregulatory factors.
- Understanding NR function is key to deciphering gene regulation.
Purpose of the Study:
- To review recent structural and proteomic advances in understanding Nuclear Receptor (NR) function.
- To elucidate how NRs interact with DNA and cofactors within chromatin.
- To provide a comprehensive overview of NR-mediated gene regulation.
Main Methods:
- X-ray crystallography to visualize NR structures and ligand interactions.
- Proteomic studies to identify NR-interacting factors.
- Analysis of NR DNA-binding and chromatin accessibility mechanisms.
Main Results:
- Detailed structural insights into NR homo- and heterodimer arrangements on DNA.
- Understanding of how ligands and coactivator peptides influence NR complexes.
- Identification of novel mechanisms for NR DNA-response element access within chromatin.
Conclusions:
- Structural and proteomic data are refining our understanding of NR complexes.
- NRs engage in diverse functional interactions with collaborating factors in chromatin.
- These findings advance our knowledge of NR-mediated gene regulation.
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