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Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

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RYBP stimulates PRC1 to shape chromatin-based communication between Polycomb repressive complexes.

Nathan R Rose¹, Hamish W King¹, Neil P Blackledge¹

¹Department of Biochemistry, University of Oxford, Oxford, United Kingdom.

|October 6, 2016

Summary

This summary is machine-generated.

Polycomb repressive complex 1 (PRC1) activity, regulated by RYBP/YAF2, shapes H2AK119 ubiquitylation. This communication with PRC2 maintains epigenetic memory for gene regulation.

Keywords:

E3 ubiquitin ligase Polycomb repressive complex chromatin chromosomes gene expression genes histone modification mouse

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Area of Science:

Epigenetics
Molecular Biology
Developmental Biology

Background:

Polycomb group (PcG) proteins are crucial chromatin-based transcriptional repressors for development.
The precise mechanisms of PcG-mediated transcriptional regulation are not fully understood.

Purpose of the Study:

To investigate the role of PRC1 catalytic subunit composition and RYBP/YAF2 in regulating H2AK119 ubiquitylation.
To elucidate the communication between PRC1 and PRC2 in establishing and maintaining Polycomb domains.

Main Methods:

Analysis of histone H2AK119 ubiquitylation in mouse embryonic stem cells.
Investigating the interplay between PRC1, PRC2, and RYBP/YAF2.
Assessing the impact of disrupted PRC1-PRC2 communication on gene expression.

Main Results:

PRC1's H2AK119 E3 ubiquitin ligase activity is composition-dependent and stimulated by RYBP/YAF2.
RYBP is essential for H2AK119 mono-ubiquitylation at PcG targets and PRC1-PRC2 communication.
Disruption of this communication leads to erosion of repressive Polycomb domains and inappropriate gene expression.

Conclusions:

RYBP-mediated communication between PRC1 and PRC2 establishes epigenetic memory through histone modifications.
This activity-based communication and threshold mechanism are vital for maintaining Polycomb chromatin domain function and gene regulation.