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Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry

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Published on: October 14, 2021

Regulatory T cells decrease invariant natural killer T cell-mediated pregnancy loss in mice.

L Li¹, J Tu¹, Y Jiang¹

¹Department of Obstetrics and Gynecology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.

Mucosal Immunology

|October 6, 2016

Summary

This summary is machine-generated.

Stimulating invariant natural killer T (iNKT) cells with α-galactosylceramide (αGC) causes pregnancy loss in mice. This occurs via interferon-gamma (IFN-γ) and impaired regulatory T (Treg) cell function.

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Last Updated: Mar 14, 2026

Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry

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Published on: October 14, 2021

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Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface

Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface

Published on: May 21, 2015

Area of Science:

Immunology
Reproductive Biology
Developmental Biology

Background:

Pregnancy loss is a common complication with poorly understood causes.
Invariant natural killer T (iNKT) cells and regulatory T (Treg) cells are implicated in reproductive outcomes.

Purpose of the Study:

To investigate the mechanisms of iNKT cell-mediated pregnancy loss.
To elucidate the role of Treg cells in this process.

Main Methods:

Mice were treated with α-galactosylceramide (αGC) to stimulate iNKT cells.
Fetal resorption, immune cell populations (iNKT, Treg), cytokine levels (IL-10, TGF-β, IFN-γ, TNF-α, IL-4), and cell co-cultures were analyzed.
Adoptive transfers of iNKT and Treg cells were performed in iNKT cell-deficient mice.

Main Results:

αGC administration induced fetal resorption, activated decidual iNKT cells, and decreased decidual Treg cells and their suppressive cytokines (IL-10, TGF-β).
Pregnancy loss was dependent on IFN-γ production by iNKT cells, as IFN-γ knockout mice were protected.
Adoptive transfer of Treg cells ameliorated αGC-induced pregnancy loss, while iNKT cells restored it in deficient mice.

Conclusions:

iNKT cell activation leads to pregnancy loss in mice, dependent on IFN-γ.
Impaired Treg cell function and reduced IL-10/TGF-β production by Treg cells are critical in iNKT cell-mediated pregnancy loss.