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Updated: Aug 17, 2026

Preparation of Meiotic Chromosome Spreads from Mouse Spermatocytes
Published on: November 22, 2017
[Various properties of DNA from isolated fractions of the mouse synaptonemal complexes]
Abstract:
A fraction of synaptonemal complexes (SC) isolated from mouse spermatocytes has been electrophoretically purified in agarose gel. The DNA from the SC fraction constitutes approximately 0.5% of total nuclear DNA, and its molecules have length heterogeneity from 1 k.b. to 20 k.b. The content of beta-globin gene is the same in DNA from the SC fraction and in total nuclear DNA. The specificity of DNA from the SC fraction is manifested by higher contents of the repeated alternative sequences GT/CA and B1-sequence that is probably due to the processes of genetic meiotic recombination.
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