Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

10.0K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.0K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

3.1K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.1K
What are Lipids?01:38

What are Lipids?

219.6K
Overview
219.6K
Lipid Digestion01:06

Lipid Digestion

99.0K
Lipids are large molecules that are generally not water-soluble. Since most of the digestive enzymes in the human body are water-based, there are specific steps the body must take to break down lipids and make them available for use.
99.0K
Structure of Lipids03:38

Structure of Lipids

98.5K
Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic...
98.5K
Autoxidation of Ethers to Peroxides and Hydroperoxides02:23

Autoxidation of Ethers to Peroxides and Hydroperoxides

9.5K
Ethers represent a class of chemical compounds that become more dangerous with prolonged storage because they tend to form explosive peroxides when standing in the air. Autoxidation is the spontaneous oxidation of a compound in air. In the presence of oxygen, ethers slowly oxidize to form hydroperoxides and dialkyl peroxides.
9.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Azimuthal separation in nearly back-to-back jet topologies in inclusive 2- and 3-jet events in <math></math> collisions at <math> </math>.

The European physical journal. C, Particles and fields·2019
Same author

Search for a Light Charged Higgs Boson Decaying to a W Boson and a CP-Odd Higgs Boson in Final States with eμμ or μμμ in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2019
Same author

Measurement of exclusive <math> </math>  photoproduction in ultraperipheral pPb collisions at <math> </math>.

The European physical journal. C, Particles and fields·2019
Same author

Search for a heavy pseudoscalar boson decaying to a Z and a Higgs boson at <math> </math>.

The European physical journal. C, Particles and fields·2019
Same author

Studies of Beauty Suppression via Nonprompt D^{0} Mesons in Pb-Pb Collisions at sqrt[s_{NN}]=5.02  TeV.

Physical review letters·2019
Same author

Study of dijet events with a large rapidity gap between the two leading jets in pp collisions at <math> </math> <math></math>.

The European physical journal. C, Particles and fields·2019

Related Experiment Video

Updated: Jan 26, 2026

A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage
07:57

A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage

Published on: May 10, 2022

5.9K

Is lipid peroxidation associated with DNA damage?

G Brambilla1, A Martelli, U M Marinari

  • 1Institute of Pharmacology, University of Genoa, Italy.

Mutation Research
|September 1, 1989
PubMed
Summary

Lipid peroxidation products showed minimal covalent binding to DNA in rat hepatocytes. Further research is needed to fully understand the DNA damage from these reactive species.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Toxicology

Background:

  • Lipid peroxidation generates reactive species that can potentially damage cellular macromolecules.
  • Understanding the interaction of these products with DNA is crucial for assessing cellular toxicity.

Purpose of the Study:

  • To investigate the specific binding of lipid peroxidation products to DNA in rat hepatocytes.
  • To quantify the extent of DNA damage induced by these reactive species.

Main Methods:

  • Rat hepatocytes were labeled with [14C(U)]arachidonic acid.
  • Cells were incubated with and without FeSO4 to induce lipid peroxidation.
  • Radioactivity bound to DNA and proteins was measured.

Main Results:

More Related Videos

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Published on: October 12, 2017

9.7K
Study of the DNA Damage Checkpoint using Xenopus Egg Extracts
10:55

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts

Published on: November 5, 2012

17.1K

Related Experiment Videos

Last Updated: Jan 26, 2026

A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage
07:57

A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage

Published on: May 10, 2022

5.9K
Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Published on: October 12, 2017

9.7K
Study of the DNA Damage Checkpoint using Xenopus Egg Extracts
10:55

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts

Published on: November 5, 2012

17.1K
  • Malondialdehyde-like substances were produced, increased by FeSO4.
  • Low but detectable radioactivity bound to DNA and proteins.
  • No direct correlation found between malondialdehyde formation and DNA/protein binding.

Conclusions:

  • Covalent binding of lipid peroxidation products to DNA in intact cells is minimal under these conditions.
  • Results suggest that DNA damage from lipid peroxidation may be less significant than previously thought.
  • Caution is advised due to potential biosynthetic incorporation of radioactivity into DNA.