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Related Experiment Videos

Major sperm protein genes from Onchocerca volvulus.

A L Scott1, J Dinman, D J Sussman

  • 1Department of Immunology and Infectious Diseases, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, MD 21205.

Molecular and Biochemical Parasitology
|September 1, 1989
PubMed
Summary

Major sperm protein (MSP) genes in nematodes show high conservation across species, indicating evolutionary stability. However, their genomic organization and intron presence vary, revealing evolutionary flexibility.

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Area of Science:

  • * Molecular Biology
  • * Parasitology
  • * Evolutionary Biology

Background:

  • * Nematode spermatozoa are unique, nonflagellated crawling cells.
  • * Major sperm protein (MSP) is a key cytoskeletal component in nematode sperm motility.
  • * MSP constitutes over 15% of sperm protein.

Purpose of the Study:

  • * To identify and characterize major sperm protein (MSP) genes in Onchocerca volvulus.
  • * To compare the genomic organization and sequence similarity of O. volvulus MSP genes with other nematode species.
  • * To investigate the evolutionary conservation and variability of MSP genes in nematodes.

Main Methods:

  • * Isolation of Onchocerca volvulus MSP clones using an Ascaris MSP cDNA probe and a lambda gt11 genomic library.

Related Experiment Videos

  • * Characterization of genomic clones (OVGS-1 and OVGS-2) via restriction endonuclease mapping and sequence analysis.
  • * Sequence comparison of O. volvulus MSP genes with Ascaris and Caenorhabditis MSP sequences.

    • Main Results:

    • * Two O. volvulus MSP genomic clones, OVGS-1 and OVGS-2, were identified and analyzed.
    • * Genes exhibit high nucleotide similarity (95%) in coding regions but lower similarity (79%) in introns.
    • * O. volvulus MSP genes show over 80% nucleotide similarity to Ascaris and 79% to Caenorhabditis MSP sequences, with high predicted amino acid similarity.

    Conclusions:

    • * Nematode MSP sequences are highly conserved throughout evolution, suggesting functional importance.
    • * Genomic organization and intron presence of MSP genes display variability among nematode species.
    • * The findings provide insights into the evolutionary dynamics of essential cytoskeletal proteins in nematodes.