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Related Concept Videos

Production of Biopesticides01:18

Production of Biopesticides

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Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...
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Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing
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A selective insecticidal protein from Pseudomonas for controlling corn rootworms.

Ute Schellenberger1, Jarred Oral1, Barbara A Rosen1

  • 1DuPont Pioneer, Hayward, CA 94545, USA.

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|October 7, 2016
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Summary
This summary is machine-generated.

A new insecticidal protein, IPD072Aa, from Pseudomonas chlororaphis effectively controls western corn rootworm (WCR) larvae. This discovery offers a vital tool against WCR resistant to current Bacillus thuringiensis (Bt) corn varieties.

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Area of Science:

  • Agricultural Entomology
  • Molecular Biology
  • Biotechnology

Background:

  • Western corn rootworm (WCR) is a major pest in North America and Europe.
  • Insect resistance to Bacillus thuringiensis (Bt) proteins in corn limits current control strategies.

Purpose of the Study:

  • To identify and characterize a novel insecticidal protein effective against WCR.
  • To evaluate the efficacy of transgenic corn expressing this new protein against WCR.

Main Methods:

  • Isolation and characterization of the insecticidal protein IPD072Aa from Pseudomonas chlororaphis.
  • Development of transgenic corn plants expressing IPD072Aa.
  • Field trials to assess protection against WCR injury.

Main Results:

  • Transgenic corn expressing IPD072Aa demonstrated significant protection against WCR damage.
  • IPD072Aa effectively killed WCR larvae, including those resistant to Bt proteins.
  • IPD072Aa showed no adverse effects on beneficial lepidopteran and hemipteran species.

Conclusions:

  • IPD072Aa represents a promising new tool for managing WCR, particularly Bt-resistant populations.
  • This protein can be utilized in transgenic corn to enhance crop protection strategies.
  • Further research may explore broader applications of IPD072Aa in pest management.