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A Visual Guide to Sorting Electrophysiological Recordings Using 'SpikeSorter'

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Sorting cells by their dynamical properties.

Ewan Henry¹, Stefan H Holm², Zunmin Zhang¹

¹Theoretical Soft Matter and Biophysics, Institute of Complex Systems and Institute for Advanced Simulation, Forschungszentrum Jülich, 52425 Jülich, Germany.

Scientific Reports

|October 7, 2016

Summary

This summary is machine-generated.

This study uses microfluidic experiments and simulations to show how cell mechanics and dynamics, not just size, can sort deformable cells like red blood cells (RBCs). Cell viscosity contrast significantly impacts RBC trajectories in deterministic lateral displacement (DLD) devices.

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Step-specific Sorting of Mouse Spermatids by Flow Cytometry

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Published on: December 31, 2015

Area of Science:

Biophysics
Microfluidics
Cellular Mechanics

Background:

Current microfluidic cell sorting often relies on size-based separation, neglecting cell deformability and complex dynamics.
Bioparticles, such as red blood cells (RBCs), are typically non-spherical and deformable, leading to intricate fluid flow behavior.
Novel cell sorting methods require sensitivity to a wider range of cellular properties beyond mere dimensions.

Purpose of the Study:

To demonstrate the utility of mechanical and dynamical properties as biomarkers for cell separation using microfluidic devices.
To investigate the influence of intra-cellular fluid viscosity and device geometry on the dynamic behavior of red blood cells (RBCs) in deterministic lateral displacement (DLD) systems.
To provide insights into the physical mechanisms governing the sorting of deformable, non-spherical cells.

Main Methods:

Combination of mesoscale hydrodynamic simulations and microfluidic experiments.
Investigation of red blood cell (RBC) dynamics within deterministic lateral displacement (DLD) devices.
Analysis of varying device geometries and viscosity contrasts between intra-cellular fluid and the suspending medium.

Main Results:

Cellular viscosity contrast and associated dynamics were found to be critical determinants of RBC trajectories within DLD devices.
Simulation results showed strong agreement with experimental data.
New understanding of the physical principles underlying the sorting of deformable, non-spherical cells was achieved.

Conclusions:

Mechanical and dynamical properties of cells can serve as effective biomarkers for advanced cell sorting applications.
The findings highlight the importance of considering cell deformability and internal dynamics in microfluidic device design.
This research paves the way for cell sorting schemes that leverage mechanics and morphology, moving beyond size-based separations.