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Bone morphogenetic protein 2 (BMP2) induces growth suppression and enhances chemosensitivity of human colon cancer

Radhakrishnan Vishnubalaji1, Shijun Yue2, Musaad Alfayez1

  • 1Stem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, Riyadh, 11461 Kingdom of Saudi Arabia.

Cancer Cell International
|October 7, 2016
PubMed
Abstract

Insights

Bone morphogenetic protein 2 (BMP2) is frequently downregulated in colorectal cancer (CRC). Restoring BMP2 inhibits CRC growth, metastasis, and sensitizes tumors to chemotherapy, suggesting a potential therapeutic strategy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Signaling

Background:

  • Molecular profiling of colorectal cancer (CRC) reveals dysregulated pathways linked to drug resistance and poor prognosis.
  • The specific role of Bone Morphogenetic Protein 2 (BMP2) signaling in CRC remains incompletely understood.

Purpose of the Study:

  • To investigate the role of BMP2 signaling in colorectal cancer progression and its potential as a therapeutic target.

Main Methods:

  • Bioinformatic analysis of the GSE21510 dataset and public gene expression data.
  • Overexpression of BMP2 in HCT116 CRC cells using lentiviral technology.
  • Gene expression profiling via microarray and qRT-PCR, cell viability assays (AlamarBlue), and in vivo studies in SCID mice.

Main Results:

  • BMP2 was frequently downregulated in primary CRC tissues and significantly lower in metastatic compared to non-metastatic cancer.
  • BMP2 overexpression altered cell cycle and DNA damage response pathways.
  • Restored BMP2 expression inhibited CRC cell growth, sphere formation, clonogenicity, migration, and sensitized cells to 5-fluorouracil (5-FU) in vitro.
  • BMP2 suppressed CRC tumor formation in vivo.

Conclusions:

  • BMP2 exhibits an inhibitory role in colorectal cancer.
  • Restoration of BMP2 expression represents a potential therapeutic strategy for CRC treatment.
Keywords:
Bone morphogenetic protein2 (BMP2)ChemosensitivityColon cancer

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