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Updated: Mar 14, 2026

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
Published on: February 3, 2021
Fluorogenic bidirectional displacement probe-based real-time isothermal DNA amplification and specific visual
Xiong Ding1, Guoping Wang1, Jingjing Sun1
1Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, P. R. China. muying@zju.edu.cn and Institute of Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, P. R. China.
Abstract:
A unique fluorogenic nucleic acid probe has been devised, called the fluorogenic bidirectional displacement probe, which can serve as both the primer and the signal indicator of amplification products for the development of the real-time isothermal DNA amplification and its visual detection of products with high sensitivity and specificity.
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