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PicoGreen: a better amyloid probe than Thioflavin-T.

Aruna K Mora¹, Prabhat K Singh¹, Birija S Patro²

¹Radiation & Photochemistry Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India. snath@barc.gov.in sukhendunath@yahoo.co.in.

Chemical Communications (Cambridge, England)

|October 7, 2016

Summary

PicoGreen, a molecular rotor, binds amyloid fibrils with high sensitivity, outperforming the standard Thioflavin-T probe. Spectroscopic and docking studies reveal its interaction mechanism with these protein aggregates.

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Area of Science:

Biochemistry
Biophysics
Molecular Biology

Background:

Amyloid fibrils are protein aggregates implicated in neurodegenerative diseases.
Thioflavin-T is the current gold standard for detecting amyloid fibrils.
There is a need for more sensitive and specific probes for amyloid detection.

Purpose of the Study:

To evaluate PicoGreen as a novel probe for amyloid fibril detection.
To compare the sensitivity of PicoGreen with Thioflavin-T.
To elucidate the interaction mechanism between PicoGreen and amyloid fibrils.

Main Methods:

Spectroscopic studies (e.g., fluorescence spectroscopy).
Molecular docking simulations.
Affinity measurements.

Main Results:

PicoGreen exhibits high affinity for amyloid fibrils.
PicoGreen demonstrates significantly higher sensitivity compared to Thioflavin-T.
Spectroscopic and docking data provide insights into the binding mode.

Conclusions:

PicoGreen is a highly sensitive and effective probe for detecting amyloid fibrils.
PicoGreen offers advantages over Thioflavin-T for amyloid detection.
Further studies are warranted to explore PicoGreen's diagnostic potential.