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Updated: Mar 14, 2026

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials
Published on: March 3, 2023
[The agar diffusion method. Round table discussion on the methods and indications for determining blood levels of
H Rosin1, H -J Hagedorn1, M Köhler1
1Institut für Medizinische Mikrobiologie und Virologie der Universität Düsseldorf, Moorenstraße 5, D-4000, Düsseldorf, Germany.
Abstract:
The present-day technique of determining the concentration of aminoglycosides in blood by agar diffusion is described including the apparatuses, set-up, and reliability tests. The results of comparative investigations showed a higher degree of conformity in Mueller-Hinton agar than in standard agar. Due to its accuracy the agar diffusion method is especially suited for the calculation of dosage recommendations and for the prediction of the maximal and minimal blood levels during treatment with aminoglycosides.
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