Related Experiment Video
Updated: Mar 14, 2026

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
Published on: September 25, 2019
Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells
Ciaran M Lee1, Haibao Zhu1, Timothy H Davis1
1Department of Bioengineering, Rice University, 6500 Main St., Houston, TX, 77030, USA.
Abstract:
The CRISPR/Cas9 system is a powerful tool for precision genome editing. The ability to accurately modify genomic DNA in situ with single nucleotide precision opens up new possibilities for not only basic research but also biotechnology applications and clinical translation. In this chapter, we outline the procedures for design, screening, and validation of CRISPR/Cas9 systems for targeted modification of coding sequences in the human genome and how to perform genome editing in induced pluripotent stem cells with high efficiency and specificity.
More Related Videos
Related Concept Videos
CRISPR/Cas9 Genome Editing
CRISPR
Induced Pluripotent Stem Cells

