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Updated: Mar 14, 2026

Homemade Site Directed Mutagenesis of Whole Plasmids
Published on: May 11, 2009
Revised Mechanism and Improved Efficiency of the QuikChange Site-Directed Mutagenesis Method
1State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, China.
Abstract:
Site-directed mutagenesis has been widely used for the substitution, addition or deletion of nucleotide residues in a defined DNA sequence. QuikChange™ site-directed mutagenesis and its related protocols have been widely used for this purpose because of convenience and efficiency. We have recently demonstrated that the mechanism of the QuikChange™ site-directed mutagenesis process is different from that being proposed. The new mechanism promotes the use of partially overlapping primers and commercial PCR enzymes for efficient PCR and mutagenesis.
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