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Related Experiment Video

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Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture
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Regulating Stem Cell Secretome Using Injectable Hydrogels with In Situ Network Formation.

Lei Cai1, Ruby E Dewi2, Andrew B Goldstone3

  • 1Department of Materials Science and Engineering, Stanford Neuroscience Institute, Stanford University, Stanford, CA, 94305, USA.

Advanced Healthcare Materials
|October 7, 2016
PubMed
Summary

New shear-thinning hydrogels (SHIELD) enable controlled stem cell delivery. Intermediate stiffness hydrogels significantly enhance the angiogenic potential of human adipose-derived stem cells for regenerative medicine.

Keywords:
angiogenic functioninjectable hydrogelsmolecular recognitionreinforcing networkstem cell secretome

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Stem Cell Biology

Background:

  • Injectable hydrogels are crucial for cell encapsulation and delivery.
  • Controlling the microenvironment is key to regulating stem cell function.
  • Stem cell secretome modulation holds therapeutic potential.

Purpose of the Study:

  • To design and synthesize shear-thinning hydrogels (SHIELD) with tunable in situ stiffening.
  • To investigate the effect of SHIELD stiffness on human adipose-derived stem cell (hASC) secretome.
  • To evaluate the impact of SHIELD-modulated hASC secretome on angiogenic potential.

Main Methods:

  • Synthesis of shear-thinning hydrogels with controlled stiffening properties.
  • Encapsulation of hASCs within hydrogels of varying stiffness.
  • Analysis of hASC secretome composition.
  • Assessment of angiogenic potential using in vitro assays.

Main Results:

  • SHIELD hydrogels exhibited controlled in situ stiffening.
  • Intermediate stiffness SHIELD (200-400 Pa) significantly altered the hASC secretome.
  • hASCs encapsulated in intermediate stiffness SHIELD demonstrated significantly promoted angiogenic potential.

Conclusions:

  • SHIELD hydrogels provide a versatile platform for injectable stem cell delivery.
  • Tunable hydrogel stiffness can effectively regulate stem cell secretome for enhanced therapeutic outcomes.
  • Intermediate stiffness SHIELD shows promise for promoting angiogenesis in regenerative applications.