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Detergent Purification of Membrane Proteins

Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...

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Related Experiment Video

Updated: Mar 14, 2026

Preparation and Friction Force Microscopy Measurements of Immiscible, Opposing Polymer Brushes

Preparation and Friction Force Microscopy Measurements of Immiscible, Opposing Polymer Brushes

Published on: December 24, 2014

Polymer Brushes for Membrane Separations: A Review.

John J Keating¹, Joseph Imbrogno¹, Georges Belfort¹

¹Department of Chemical and Biological Engineering and Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute , Troy, New York 12180, United States.

ACS Applied Materials & Interfaces

|October 7, 2016

Summary

This summary is machine-generated.

Polymer brush-modified membranes offer tunable performance for diverse applications. Grafting polymer brushes enhances membrane capabilities in separations like water purification and gas separation.

Keywords:

desalination gas separation pervaporation polymer brush protein adsorption stimuli responsive surface modification synthetic membrane

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Area of Science:

Materials Science
Chemical Engineering
Membrane Science

Background:

Polymer brush-modified membranes represent an advanced class of synthetic membranes.
Tuning membrane performance is achieved through polymer brush grafting techniques.

Purpose of the Study:

To review the fundamentals and applications of polymer brush-modified membranes.
To highlight the performance characteristics of these membranes in various separation processes.

Main Methods:

Review of literature on polymer brush grafting onto membranes.
Analysis of performance data across different separation applications.

Main Results:

Polymer brushes provide a versatile method for designing advanced membranes.
Demonstrated effectiveness in protein adsorption, colloid stabilization, sensors, water purification, pervaporation, and gas separations.

Conclusions:

Polymer brush modification is a key strategy for developing high-performance membranes.
These membranes show significant potential in a wide array of scientific and industrial applications.