Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Conjugable, Antifouling, and Non-immunogenic Coatings for Gold Nanoparticles by Multivalent Grafting of Azide-Bearing Polyoxazoline Brushes.

Langmuir : the ACS journal of surfaces and colloids·2026

Same author

Self-Controlled Automated Strategy for the Synthesis of Gold Nanorods With Fine-Tuned Longitudinal Absorption.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026

Same author

Near-Quantitative Formation of Imines in Water with Allosteric Control.

Journal of the American Chemical Society·2026

Same author

Function from Confinement: Ligand-Coated Nanoparticles as Functional Materials.

ACS nano·2025

Same author

Heavy Alkaline Earth Radiometals for Cancer Theranostics: Coordination and Radiochemistry of Radium-223 and Barium-131 with Kryptofix 22-Based Chelators.

Inorganic chemistry·2025

Same author

A Scoring Function for Monolayer-Protected Gold Nanoparticles Capable of Recognizing Small Organic Molecules in Solution.

Journal of chemical theory and computation·2025

Search research articles

Related Experiment Video

Updated: Mar 14, 2026

Detection and Removal of Tooth-Colored Composite Resin Using the Fluorescence-Aided Identification Technique

Detection and Removal of Tooth-Colored Composite Resin Using the Fluorescence-Aided Identification Technique

Published on: July 27, 2022

Minimal Self-Immolative Probe for Multimodal Fluoride Detection.

Luca Gabrielli¹, Fabrizio Mancin¹

¹Università di Padova , Dipartimento di Scienze Chimiche, via Marzolo 1, 35125 Padova, Italy.

The Journal of Organic Chemistry

|October 7, 2016

Summary

New self-immolative fluoride probes offer simple preparation and naked-eye detection of fluoride ions. Their reactivity is influenced by the positioning of protective groups, impacting the fluoride ion release rate.

More Related Videos

µTongue: A Microfluidics-Based Functional Imaging Platform for the Tongue In Vivo

µTongue: A Microfluidics-Based Functional Imaging Platform for the Tongue In Vivo

Published on: April 22, 2021

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Published on: January 5, 2015

Related Experiment Videos

Last Updated: Mar 14, 2026

Detection and Removal of Tooth-Colored Composite Resin Using the Fluorescence-Aided Identification Technique

Detection and Removal of Tooth-Colored Composite Resin Using the Fluorescence-Aided Identification Technique

Published on: July 27, 2022

µTongue: A Microfluidics-Based Functional Imaging Platform for the Tongue In Vivo

µTongue: A Microfluidics-Based Functional Imaging Platform for the Tongue In Vivo

Published on: April 22, 2021

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Published on: January 5, 2015

Area of Science:

Chemical sensing
Organic synthesis
Analytical chemistry

Background:

Fluoride ion detection is crucial in various fields.
Existing fluoride probes often have complex structures and synthesis.
There is a need for simpler, more accessible fluoride sensing systems.

Purpose of the Study:

To develop novel single-molecule, self-immolative probes for fluoride ion detection.
To investigate the structure-reactivity relationship of these new probes.
To enable simple and effective fluoride detection at micromolar concentrations.

Main Methods:

Synthesis of tert-butyldimethylsilyl-protected 2- and 4-difluoromethylphenol probes.
Fluoride ion detection using naked-eye observation, UV-vis absorption, and fluorescence spectroscopy.
Kinetic studies to elucidate the self-immolative reaction mechanism.

Main Results:

Two novel self-immolative fluoride probes were synthesized via a straightforward two-step procedure.
The probes enable naked-eye, UV-vis, and fluorescence detection of fluoride ions at micromolar levels.
The rate-limiting step was identified as the initial fluoride ion release.
The positional isomerism of the protecting groups significantly affects probe reactivity.

Conclusions:

The developed probes offer a simpler and more efficient alternative for fluoride detection.
Understanding the reaction mechanism provides insights for designing future sensing molecules.
The probes demonstrate potential for practical applications requiring sensitive fluoride ion monitoring.