Integrated Left Ventricular Global Transcriptome and Proteome Profiling in Human End-Stage Dilated Cardiomyopathy

Area of Science:

• Cardiovascular Research
• Molecular Biology
• Biomarker Discovery

Background:

• Idiopathic dilated cardiomyopathy (DCM) pathogenesis remains unclear.
• Understanding molecular changes is crucial for biomarker discovery and disease management.

Purpose of the Study:

• To investigate integrated global transcriptional and translational changes in human DCM.
• To identify novel disease biomarkers and pathways associated with DCM.

Main Methods:

• Compared myocardial tissues from five DCM hearts and five non-failing (NF) donor hearts.
• Utilized high-density oligonucleotide microarrays for transcriptome profiling.
• Employed liquid chromatography-tandem mass spectrometry for proteome expression analysis.

Main Results:

• Identified 1262 differentially expressed genes (DEGs) and 269 differentially expressed proteins (DEPs).
• Found significant overlap in pathways related to metabolism, inflammation, and ATP synthesis.
• Highlighted sixteen commonly altered entities between transcriptome and proteome analyses.

Conclusions:

• Integrated transcriptome and proteome analysis revealed novel insights into DCM.
• Identified sixteen commonly altered entities and novel genes/proteins/pathways.
• Data provides a foundation for improved DCM management strategies.