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Liquid-liquid extraction of enzymatically synthesized functional RNA oligonucleotides using reverse micelles with a
Tatsuo Maruyama1, Naoki Ishizu1, Yuka Eguchi1
1Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan. tmarutcm@crystal.kobe-u.ac.jp.
Abstract:
We successfully implemented solvent extraction of short, single-stranded RNA using reverse micelles (water-in-oil microemulsions) with a DNA-surfactant. A thrombin-binding RNA aptamer was enzymatically synthesized and purified by extraction using the reverse micellar system. The extracted RNA aptamer retained thrombin-binding activity after the extraction procedure.
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