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Updated: Mar 14, 2026
Selective Capture of 5-hydroxymethylcytosine from Genomic DNA
Published on: October 5, 2012
Simultaneous and Sensitive Detection of Multisite 5-Methylcytosine Including Non-CpG Sites at Single-5mC-Resolution
Shuang Rao1, Yuqi Chen1, Tingting Hong1
1College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University , Wuhan 430072, Hubei Province, China.
Abstract:
The methylation status of multiplexed methylcytosine sites can be simultaneously monitored by ligation-depended PCR assay. The ability of quantitative detection of multiplexed sites in one PCR reaction makes it a good choice for detecting methylation at both CpG and non-CpG sites for research and diagnosis of disease compared with others. The assay can determine as low as 20 aM methylated DNA and has been successfully applied to the genomic DNA sample derived from cancer cell lines.
