Related Experiment Video

Updated: Mar 14, 2026

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry
09:41

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Published on: December 28, 2021

2.3K

Spc24 is required for meiotic kinetochore-microtubule attachment and production of euploid eggs

Teng Zhang1,2, Yang Zhou2,3, Hong-Hui Wang2

  • 1Institute of Reproductive Sciences, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.

Oncotarget
|October 8, 2016
PubMed

Insights

Spc24 protein is crucial for accurate chromosome segregation in mammalian oocytes. Its depletion disrupts kinetochore-microtubule attachments, leading to aneuploidy and impacting genomic stability during meiosis.

Area of Science:

  • Cell Biology
  • Genetics
  • Reproductive Biology

Background:

  • Mammalian oocytes exhibit high error rates in chromosome segregation during meiosis.
  • Faithful chromosome segregation relies on proper kinetochore-microtubule attachments.

Purpose of the Study:

  • To investigate the role of Spc24 in mouse oocyte meiosis.
  • To understand Spc24's contribution to kinetochore function and genomic stability.

Main Methods:

  • Localization of Spc24 at kinetochores during mouse oocyte meiosis.
  • siRNA-mediated depletion of Spc24.
  • Analysis of kinetochore-microtubule attachments and chromosome alignment.
  • Assessment of spindle assembly checkpoint (SAC) protein Mad2 recruitment.

Main Results:

  • Spc24 localizes to kinetochores in mouse oocytes during meiosis.
  • Spc24 depletion results in defective kinetochore-microtubule attachments and chromosome misalignment.
  • Spc24 depletion abrogates Mad2 recruitment, accelerates meiosis I, and increases aneuploidy incidence.

Conclusions:

  • Spc24 is essential for maintaining kinetochore-microtubule attachment fidelity during oocyte meiosis.
  • Spc24 plays a critical role in ensuring genomic stability by regulating the spindle assembly checkpoint.
Keywords:
Spc24aneuploidykinetochore-microtubule attachmentmeiosisoocyte

More Related Videos

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes10:09

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

Published on: September 13, 2022

2.7K
A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model09:40

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

16.0K

Related Concept Videos

The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.9K
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

1.8K
Meiosis II02:02

Meiosis II

Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
51.4K
Meiosis II01:57

Meiosis II

Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each...
209.9K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
4.6K
Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall...
4.2K