Related Experiment Video
Updated: Mar 14, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
Published on: October 7, 2025
Label-Free Isothermal Amplification Assay for Specific and Highly Sensitive Colorimetric miRNA Detection
Stefano Persano1, Maria L Guevara2, Joy Wolfram2
1Department of Nanomedicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston 77030, Texas, United States; Istituto Italiano di Tecnologia (IIT), Via Morego, 30, 16163 Genova, Italy; Università del Salento, Via Provinciale Monteroni, 73100 Lecce, Italy.
Abstract:
We describe a new method for the detection of miRNA in biological samples. This technology is based on the isothermal nicking enzyme amplification reaction and subsequent hybridization of the amplification product with gold nanoparticles and magnetic microparticles (barcode system) to achieve naked-eye colorimetric detection. This platform was used to detect a specific miRNA (miRNA-10b) associated with breast cancer, and attomolar sensitivity was demonstrated. The assay was validated in cell culture lysates from breast cancer cells and in serum from a mouse model of breast cancer.

