Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A novel biomimetic opto-electronic nose combining cross-reactive peptides with selective phage display peptides for enhanced performance.

Biosensors & bioelectronics·2026
Same author

Compact RNA sensors for increasingly complex functions of multiple inputs.

Nature chemistry·2025
Same author

Au@mSiO<sub>2</sub> nanocomposites with large pores for protein transport.

Journal of materials chemistry. B·2025
Same author

Study and optimization of the selectivity of an odorant binding protein-based bioelectronic nose.

Biosensors & bioelectronics·2024
Same author

Data Processing for Predicting DNA Damaging Properties of Complex UV Sources.

Chemphyschem : a European journal of chemical physics and physical chemistry·2024
Same author

Investigation of the Affinity of Aptamers for Bacteria by Surface Plasmon Resonance Imaging Using Nanosomes.

ACS applied materials & interfaces·2024
Same journal

Curved interfaces-enhanced oxygen reduction reaction by PtCo alloys anchored MOF-derived carbon.

Nanoscale·2026
Same journal

Broadly neutralizing antibodies against HIV-1 pseudoviruses elicited by envelope trimer DNA with chimeric design delivered <i>via</i> silica-calcium phosphate nanoparticles.

Nanoscale·2026
Same journal

The transition of MXene research: the map and the gap.

Nanoscale·2026
Same journal

Critical interplay of defect engineering and plasmonics in hybrid nanostructures for ultrasensitive photo-enhanced Raman spectroscopy.

Nanoscale·2026
Same journal

Crystallization regulation and electrochemical optimization of free-standing carbon nanofiber-confined vanadium oxide nanodots for advanced flexible zinc ion batteries.

Nanoscale·2026
Same journal

Polariton manipulation <i>via</i> boundary engineering.

Nanoscale·2026
See all related articles

Related Experiment Video

Updated: Mar 14, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

11.3K

A nanoparticle-based thermo-dynamic aptasensor for small molecule detection.

Feriel Melaine1, Clothilde Coilhac1, Yoann Roupioz1

  • 1Université Grenoble Alpes, INAC, SPrAM, F-38000 Grenoble, France. arnaud.buhot@cea.fr and CEA, INAC, SPrAM, F-38000 Grenoble, France and CNRS, SPrAM, F-38000 Grenoble, France.

Nanoscale
|October 8, 2016
PubMed
Summary
This summary is machine-generated.

We developed a novel aptasensor for detecting small molecules like adenosine. This biosensor uses engineered split-aptamer sequences and gold nanoparticles (AuNPs) for enhanced sensitivity, achieving detection limits 200 times lower than traditional methods.

More Related Videos

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.7K
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

10.4K

Related Experiment Videos

Last Updated: Mar 14, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

11.3K
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.7K
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

10.4K

Area of Science:

  • Biomolecular Engineering
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Small molecules are crucial biomarkers, but their detection often requires sensitive methods.
  • Aptamers, short nucleic acid strands, offer specific molecular recognition for biosensor development.
  • Adenosine-specific aptamers exhibit high affinity for adenosine detection (KD = 6 μM).

Purpose of the Study:

  • To demonstrate the feasibility of an aptasensor for small molecule detection using engineered split-aptamers.
  • To improve the limit of detection (LOD) for adenosine compared to native aptamers.
  • To integrate surface plasmon resonance imaging and gold nanoparticles for signal amplification.

Main Methods:

  • Engineering split-aptamer sequences for thermodynamic monitoring of target binding.
  • Utilizing surface plasmon resonance imaging (SPRi) on micro-arrays for detection.
  • Conjugating gold nanoparticles (AuNPs) to aptamer fragments for signal enhancement.

Main Results:

  • Achieved proof-of-principle for the split-aptamer based aptasensor.
  • Demonstrated a significant signal amplification through DNA sequence engineering and AuNP conjugation.
  • Reached a limit of detection (LOD) of 30 nM for adenosine, which is 200 times lower than the native aptamer's KD.

Conclusions:

  • The developed aptasensor offers a highly sensitive and specific method for small molecule detection.
  • Engineered split-aptamers combined with AuNPs and SPRi represent a powerful strategy for advanced biosensing.
  • This approach significantly enhances biomarker detection capabilities for small molecules.