Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluating the utility of a patient and public involvement and engagement (PPIE) end-of-trial event to re-engage with cell-based therapy participants.

Regenerative medicine·2025
Same author

In vitro model of neurotrauma using the chick embryo to test regenerative bioimplantation.

ALTEX·2023
Same author

Systematic Alignment Analysis of Neural Transplant Cells in Electrospun Nanofibre Scaffolds.

Materials (Basel, Switzerland)·2023
Same author

A benchtop brain injury model using resected donor tissue from patients with Chiari malformation.

Neural regeneration research·2022
Same author

Electroactive Scaffolds to Improve Neural Stem Cell Therapy for Spinal Cord Injury.

Frontiers in medical technology·2022
Same author

Design and psychometric testing of a new patient-reported outcome measure for ankle treatment.

Foot (Edinburgh, Scotland)·2021

Related Experiment Video

Updated: Mar 14, 2026

Fabrication of Magnetic Platforms for Micron-Scale Organization of Interconnected Neurons
09:54

Fabrication of Magnetic Platforms for Micron-Scale Organization of Interconnected Neurons

Published on: July 14, 2021

5.3K

Nanoengineering neural stem cells on biomimetic substrates using magnetofection technology.

Christopher F Adams1, Andrew W Dickson2, Jan-Herman Kuiper3

  • 1Institute of Science and Technology in Medicine, Keele University, Newcastle-under-Lyme, ST5 5BG, UK. c.adams@keele.ac.uk.

Nanoscale
|October 8, 2016
PubMed
Summary

This study introduces magnetofection for genetically engineering neural stem cells (NSCs) in biomimetic hydrogels. Magnetic field application enhances MNP transfection in 3D NSC spheroids, improving neural tissue engineering relevance.

More Related Videos

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles
08:26

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

Published on: October 19, 2015

12.8K
3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration
09:46

3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration

Published on: April 27, 2017

10.4K

Related Experiment Videos

Last Updated: Mar 14, 2026

Fabrication of Magnetic Platforms for Micron-Scale Organization of Interconnected Neurons
09:54

Fabrication of Magnetic Platforms for Micron-Scale Organization of Interconnected Neurons

Published on: July 14, 2021

5.3K
Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles
08:26

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

Published on: October 19, 2015

12.8K
3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration
09:46

3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration

Published on: April 27, 2017

10.4K

Area of Science:

  • Biomaterials Science
  • Nanomedicine
  • Neuroscience

Background:

  • Tissue engineering increasingly uses biomimetic materials to mimic native tissue properties.
  • Neural cell studies, especially in nanomedicine, lag in using neuromimetic substrates.
  • Existing magnetic nanoparticle (MNP) applications for neural cells use non-physiological substrates.

Purpose of the Study:

  • To test magnetofection for genetically engineering neural stem cells (NSCs) in biomimetic hydrogels.
  • To evaluate the utility of magnetic field-enhanced MNP delivery in 3D NSC cultures.
  • To compare NSC membrane activity on soft biomimetic versus hard non-neuromimetic substrates.

Main Methods:

  • Neural stem cells (NSCs) cultured in 3D collagen hydrogels (biomimetic) and on hard substrates.
  • Magnetofection technique utilizing magnetic nanoparticles (MNPs) and applied magnetic fields.
  • High-resolution scanning electron microscopy (SEM) to assess NSC membrane activity and MNP uptake via endocytosis.

Main Results:

  • Magnetic field application safely enhanced MNP-mediated transfection of NSCs in 3D spheroid structures.
  • Biomimetic hydrogels better replicate neural tissue's mechanical and structural properties compared to hard substrates.
  • NSCs exhibited lower membrane activity on soft substrates than on hard ones, impacting MNP uptake.

Conclusions:

  • Magnetofection is a viable strategy for genetically engineering NSCs within physiologically relevant biomimetic environments.
  • Culturing NSCs in neuromimetic hydrogels offers greater translational and physiological relevance for nanomedicine applications.
  • Understanding NSC membrane dynamics on different substrates is crucial for optimizing MNP-mediated genetic engineering strategies.