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09:59
Preparation of Exosomes for siRNA Delivery to Cancer Cells
Published on: December 5, 2018
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Programmable RNA microstructures for coordinated delivery of siRNAs.
Jaimie Marie Stewart1, Mathias Viard2, Hari K K Subramanian3
1Department of Bioengineering, University of California, Riverside, Riverside, CA 92521, USA.
Nanoscale
|October 8, 2016
Summary
Researchers developed large, micron-size RNA scaffolds for synchronized drug delivery. These RNA lattices show enhanced stability and efficiently deliver therapeutic siRNAs to cancer cells, advancing RNA nanotechnology for disease treatment.
Area of Science:
- Biotechnology and Nanotechnology
- Molecular Biology and Genetics
- Therapeutic Delivery Systems
Background:
- Artificial self-assembling RNA nanostructures are crucial for drug delivery and metabolic regulation.
- Current RNA scaffolds are nanosize, limiting ligand arrangement and cargo concentration.
- Larger RNA scaffolds are needed to enhance therapeutic delivery capabilities.
Purpose of the Study:
- To investigate the assembly and functionalization of micron-size RNA scaffolds for synchronized cargo delivery.
- To explore the potential of these larger RNA structures for therapeutic applications in cancer cells.
Main Methods:
- Adapted a double crossover (DX) DNA tile motif to design de novo DX RNA tiles.
- Optimized assembly protocols for high-yield RNA lattice formation.
- Functionalized RNA lattices with small interfering RNAs (siRNAs) and fluorescent tags for cargo delivery.
Main Results:
- Successfully assembled robust and modular micron-size RNA lattices.
- Demonstrated efficient gene knockdown in human breast and prostate cancer cells via RNA lattice transfection.
- RNA lattices exhibited superior stability in blood serum compared to individual RNA tiles.
Conclusions:
- Micron-size RNA scaffolds can be designed and assembled using a DNA tiling approach.
- These RNA lattices are effective for synchronized delivery of therapeutic cargos like siRNAs.
- The enhanced stability and modularity of RNA lattices position them as promising tools for therapeutic RNA nanotechnology.
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