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Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...

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Related Experiment Video

Updated: Mar 14, 2026

Derivation of Highly Purified Cardiomyocytes from Human Induced Pluripotent Stem Cells Using Small Molecule-modulated Differentiation and Subsequent Glucose Starvation

Derivation of Highly Purified Cardiomyocytes from Human Induced Pluripotent Stem Cells Using Small Molecule-modulated Differentiation and Subsequent Glucose Starvation

Published on: March 18, 2015

A Glycolytic Solution for Pluripotent Stem Cells.

Barbara Mlody¹, Alessandro Prigione¹

¹Max Delbrueck Center for Molecular Medicine (MDC), D-13125 Berlin, Germany.

|October 8, 2016

Summary

This summary is machine-generated.

Glycolysis, a key metabolic pathway, increases in human pluripotent stem cells (PSCs) during specific culture conditions or state transitions. This dynamic flux supports stem cell function and maintenance.

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Last Updated: Mar 14, 2026

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Area of Science:

Cellular Metabolism
Stem Cell Biology
Biochemistry

Background:

Glycolysis is a fundamental metabolic pathway crucial for cellular energy production.
Pluripotent stem cells (PSCs) exhibit unique metabolic characteristics.
Understanding PSC metabolism is vital for regenerative medicine and developmental biology.

Purpose of the Study:

To investigate the dynamic changes in glycolytic flux in human pluripotent stem cells (PSCs).
To explore the impact of culture conditions and state transitions on PSC glycolysis.
To elucidate the role of glycolysis in maintaining PSC pluripotency.

Main Methods:

Analysis of glycolytic flux in human primed PSCs.
Comparison of metabolic profiles under feeder-free cultivation.
Assessment of metabolic changes during conversion to the naive pluripotent state.

Main Results:

Glycolytic flux is dynamically increased in human primed PSCs.
Feeder-free cultivation leads to enhanced glycolytic activity.
Conversion to the naive state is associated with increased glycolytic flux.

Conclusions:

Glycolysis plays a dynamic and essential role in human pluripotent stem cell metabolism.
Culture conditions and state transitions significantly modulate PSC glycolytic flux.
These findings provide insights into the metabolic regulation of stem cell pluripotency.