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Related Experiment Video

Updated: Mar 14, 2026

Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts

Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts

Published on: February 5, 2018

Generating human serotonergic neurons in vitro: Methodological advances.

Krishna C Vadodaria¹, Maria C Marchetto¹, Jerome Mertens¹

¹Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, CA, USA.

Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology

|October 8, 2016

Summary

New methods efficiently generate human serotonergic neurons in vitro. These neurons produce serotonin, are electrically active, and respond to SSRIs, aiding neurotransmission research and disease modeling.

Keywords:

SSRI antidepressants direct conversion iPSC neuropsychiatric disorders pluripotent stem cells serotonergic differentiation

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Last Updated: Mar 14, 2026

Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts

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Published on: February 5, 2018

Production of Human Neurogenin 2-Inducible Neurons in a Three-Dimensional Suspension Bioreactor

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Published on: March 17, 2023

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Published on: October 28, 2011

Area of Science:

Neuroscience
Stem Cell Biology
Developmental Biology

Background:

Human neurotransmission research is advanced by in vitro neuron models.
Serotonergic neurons are crucial for mood regulation and neurological functions.

Purpose of the Study:

To review recent advancements in generating human serotonergic neurons in vitro.
To compare different methodologies for serotonergic neuron differentiation.
To discuss the utility of these models for in vitro disease modeling.

Main Methods:

Overview of transdifferentiation techniques overexpressing transcription factors.
Description of induced pluripotent stem cell (iPSC) differentiation using developmental cues.
Analysis of in vitro functional characteristics of derived neurons.

Main Results:

Multiple methods efficiently produce functional human serotonergic neurons in vitro.
Derived neurons exhibit serotonin production, express key raphe genes, and show electrical activity.
Neurons respond to selective serotonin reuptake inhibitors (SSRIs) in vitro.

Conclusions:

Recent methods provide robust tools for studying serotonergic neurotransmission in vitro.
These human serotonergic neuron models are valuable for in vitro disease modeling.
Harnessing developmental programs enables efficient in vitro serotonergic neuron differentiation.