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Development of Analytical Methods

Development of Analytical Methods

An analytical methodology can be divided into four sequential steps: technique, method, procedure, and protocol. A technique is a scientific principle that rationalizes a specific phenomenon through chemical measurements. Adapting a technique for analyzing a sample of interest is termed a method. The procedure outlines the directions for performing the analysis via an analytical method. The protocol is the detailed guidelines on the procedure, which should be strictly followed to obtain the...

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Related Experiment Video

Updated: Mar 14, 2026

Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor

Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor

Published on: January 12, 2019

Progress and development of analytical methods for gibberellins.

Chaozhi Pan¹, Swee Ngin Tan², Jean Wan Hong Yong³

¹Advanced Environmental Biotechnology Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, Singapore, Singapore.

Journal of Separation Science

|October 8, 2016

Summary

This review details analytical methods for gibberellins, plant hormones crucial for crop yield. It covers extraction, purification, and high-throughput techniques like chromatography and mass spectrometry for accurate plant hormone analysis.

Keywords:

Analytical methods Gibberellins Phytohormones Sample preparation

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Last Updated: Mar 14, 2026

Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor

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Published on: January 12, 2019

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Quantification of Endogenous Auxin and Cytokinin During Internode Culture of Ipecac

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Published on: March 15, 2018

Area of Science:

Plant Science
Analytical Chemistry

Background:

Gibberellins are vital phytohormones regulating plant growth and development, impacting crop yields.
Accurate quantification of gibberellins in plant tissues is essential but challenging due to their trace amounts.

Purpose of the Study:

To provide a comprehensive review of analytical methods for gibberellin determination.
To critically evaluate techniques for analyzing gibberellins in complex plant matrices.
To discuss the advantages and limitations of various analytical approaches.

Main Methods:

Detailed review of sample pre-treatment steps: extraction, pre-concentration, and purification.
Focus on high-throughput analytical methods including gas chromatography, liquid chromatography, and capillary electrophoresis.
Emphasis on hyphenated techniques, particularly mass spectrometry, for sensitive and specific detection.

Main Results:

Discussion of various analytical methods suitable for complex plant matrices.
Evaluation of the strengths and weaknesses of different chromatographic and electrophoretic techniques.
Identification of trends in high-throughput gibberellin analysis.

Conclusions:

Effective analytical strategies are crucial for understanding gibberellin roles in plants.
Advancements in chromatography and mass spectrometry enable precise gibberellin quantification.
Future trends point towards more efficient and sensitive methods for plant hormone analysis.