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Ferritin-supported lipid bilayers for triggering the endothelial cell response.
C Satriano1, G Lupo2, C Motta2
1Department of Chemical Sciences, University of Catania, Viale Andrea Doria, 6, 95125 Catania, Italy.
Colloids and Surfaces. B, Biointerfaces
|October 9, 2016
Summary
Hybrid nanoassemblies of ferritin and silica-supported lipid bilayers (ferritin-SLBs) enhance retinal endothelial cell (EC) adhesion and proliferation. The optimal POPC/POEPC ratio of 75:25 significantly boosted cell growth, showing promise for tissue engineering applications.
Area of Science:
- Biomaterials Science
- Cell Biology
- Nanotechnology
Background:
- Endothelial cells (ECs) are crucial for retinal vascular health.
- Developing biomaterials that promote EC adhesion and proliferation is vital for regenerative medicine.
- Ferritin-based nanoassemblies offer unique properties for cell-material interactions.
Purpose of the Study:
- To investigate the impact of ferritin-silica-supported lipid bilayers (ferritin-SLBs) on retinal microvascular endothelial cell (EC) behavior.
- To explore how varying lipid compositions and surface charges of SLBs influence EC adhesion and proliferation.
- To assess the role of ferritin in modulating EC responses on these biomaterial platforms.
Main Methods:
- Preparation of hybrid nanoassemblies using ferritin and silica-supported lipid bilayers (SLBs) with varying lipid ratios (POPC/POEPC).
- Characterization of ferritin-SLB formation, interaction kinetics, and viscoelastic properties using quartz crystal microbalance with dissipation monitoring and atomic force microscopy.
- Evaluation of EC adhesion, spreading, and proliferation on ferritin-SLB substrates via phase-contrast optical microscopy.
Main Results:
- The POPC/POEPC (75:25) lipid ratio demonstrated the highest EC adhesion (2-4h) and proliferation (24h-5 days).
- Ferritin significantly enhanced EC adhesion (1.5-fold) and proliferation (1.75-fold) compared to bare glass.
- Ferritin further boosted proliferation (2-fold) compared to bare POPC/POEPC (95:5) SLBs.
Conclusions:
- Hybrid ferritin-SLB nanoassemblies can effectively modulate endothelial cell responses.
- The surface charge and viscoelastic properties of SLBs, influenced by lipid composition, are key factors in cell interaction.
- These findings highlight the potential of ferritin-SLBs as promising platforms for promoting retinal EC adhesion and proliferation in regenerative medicine.
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