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Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Mass Spectrometry: Overview01:19

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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Mass Spectrometry: Complex Analysis01:21

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Updated: Mar 13, 2026

Analyzing Large Protein Complexes by Structural Mass Spectrometry
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Mass spectrometry guided structural biology.

Idlir Liko1, Timothy M Allison1, Jonathan Ts Hopper1

  • 1Physical and Theoretical Chemistry Laboratory, South Parks Road, Oxford, OX1 3QZ, United Kingdom.

Current Opinion in Structural Biology
|October 11, 2016
PubMed
Summary

Mass spectrometry enhances structural biology by defining protein complex stoichiometry and molecular details. It also optimizes conditions for determining structures of difficult biomolecules.

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Area of Science:

  • Structural biology
  • Biophysics
  • Biochemistry

Background:

  • Advancements in cryo-electron microscopy and crystallography are improving structural resolution.
  • Emerging interfaces between biophysical methods offer new avenues for biomolecular analysis.

Purpose of the Study:

  • To explore how mass spectrometry (MS) can enhance structural biology techniques.
  • To highlight MS's role in defining subunit stoichiometry and molecular details.
  • To demonstrate MS's utility in optimizing conditions for structure determination.

Main Methods:

  • Utilizing high-resolution mass spectrometry for subunit stoichiometry.
  • Applying advanced mass spectral resolution to interpret complex structural data.
  • Employing MS to identify optimal solution conditions for biomolecular studies.

Main Results:

  • Mass spectrometry provides unambiguous definition of subunit stoichiometry in protein complexes.
  • Increased mass spectral resolution allows detailed molecular interpretation of unassigned densities in structural maps.
  • Mass spectrometry effectively guides the selection of solution conditions for challenging structure determination.

Conclusions:

  • Mass spectrometry is a powerful tool for advancing structural biology.
  • MS integration with cryo-electron microscopy and crystallography yields deeper insights into protein complex structure.
  • MS facilitates the structure determination of recalcitrant biomolecules.