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Updated: Mar 13, 2026
Author Spotlight: Unlocking the Mysteries of Oral Potential Malignancies
Published on: August 11, 2023
Stromal fibers in oral squamous cell carcinoma: A possible new prognostic indicator?
Priyanka Kardam1, Monica Mehendiratta1, Shweta Rehani1
1Department of Oral Pathology and Microbiology, Sudha Rustagi College of Dental Sciences and Research, Faridabad, Haryana, India.
Background:
Many studies have been carried out to study the role of extracellular matrix proteins, growth factors and matrix metalloproteinases on tumor invasion. However, literature related to the analysis of connective tissue fibers in varying grades of oral squamous cell carcinoma (OSCC) is very limited.
Aim:
To analyze the changes in collagen and elastic fibers in varying grades of (OSCC).
Settings And Design:
This retrospective study was carried out using a light and polarizing microscope.
Materials And Methods:
Three sections each were cut from fifty samples of varying grades of OSCC and ten samples of control followed by staining with H and E, Picrosirius-Red and Verhoeff-Van Gieson. Qualitative and quantitative analysis of collagen and elastic fibers were accomplished using set criteria.
Statistical Analysis:
Data were entered into the Statistical Package for Social Sciences (SPSS) version 13.5 for analysis.
Results:
A change in colors of collagen fibers was seen on progressing from well to poorly differentiated OSCC. Thin collagen fibers predominantly exhibited greenish yellow, but the thick fibers exhibited a variety of colors. As the grade of OSCC progressed, collagen fibers were loosely packed haphazardly arranged. Statistically insignificant results were obtained for quantitative analysis of collagen and qualitative analysis of elastic fibers.
Conclusion:
The collagen fibers undergo a change in color, orientation and packing in the stroma of varying grades of OSCC. The uniqueness of this study lies in the exploration of elastic fibers in OSCC which has not been done so far.
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