Related Experiment Video
Updated: Mar 13, 2026

05:37
Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
Published on: April 4, 2025
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Studies of G-quadruplexes formed within self-assembled DNA mini-circles
Beata Klejevskaja1, Alice L B Pyne2, Matthew Reynolds3
1Department of Chemistry, Imperial College London, London, SW7 2AZ, UK. r.vilar@imperial.ac.uk and Institute of Chemical Biology, Imperial College London, London, SW7 2AZ, UK. l.ying@imperial.ac.uk.
Abstract
None:
We have developed self-assembled DNA mini-circles that contain a G-quadruplex-forming sequence from the c-Myc oncogene promoter and demonstrate by FRET that the G-quadruplex unfolding kinetics are 10-fold slower than for the simpler 24-mer G-quadruplex that is commonly used for FRET experiments.

