Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

3D killing assay of cancer spheroids by cytotoxic T lymphocytes in anchored microfluidic droplets.

Methods in cell biology·2026
Same author

Controlling the Collective Transport of Large Passive Particles With Suspensions of Microorganisms.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

SPEF1 mediates assembly of the central pair microtubule complexes in cilia of <i>Tetrahymena</i>.

bioRxiv : the preprint server for biology·2026
Same author

Spherical Skin Model: Stratified Co-Culture of Fibroblasts and Keratinocytes on Spherical Beads Toward Compound Screening.

Advanced healthcare materials·2025
Same author

A Marangoni swimmer pushing a particle raft under 1D confinement.

Soft matter·2025
Same author

Culture of pluripotent stem cells in microscale droplets modulates differentiation and tissue patterning towards organoids on chip.

Stem cell research & therapy·2025

Related Experiment Video

Updated: Mar 13, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

23.0K

Universal microfluidic platform for bioassays in anchored droplets.

Gabriel Amselem1, Cyprien Guermonprez1, Benoît Drogue1

  • 1LadHyX and Department of Mechanics, Ecole Polytechnique, CNRS, 91128 Palaiseau, France. baroud@ladhyx.polytechnique.fr.

Lab on a Chip
|October 11, 2016
PubMed
Summary

This study introduces a versatile droplet microfluidic platform that mimics multiwell plates for biological research. The system enables easy cell encapsulation, monitoring, and content extraction, simplifying complex experiments for broader lab adoption.

More Related Videos

Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation
08:27

Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation

Published on: August 28, 2017

5.8K
Particle Templated Emulsification enables Microfluidic-Free Droplet Assays
11:03

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

Published on: March 9, 2021

6.9K

Related Experiment Videos

Last Updated: Mar 13, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

23.0K
Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation
08:27

Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation

Published on: August 28, 2017

5.8K
Particle Templated Emulsification enables Microfluidic-Free Droplet Assays
11:03

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

Published on: March 9, 2021

6.9K

Area of Science:

  • Microfluidics
  • Biotechnology
  • Cell Biology

Background:

  • Droplet-based microfluidics offer powerful tools but face limited adoption in non-specialist labs due to complexity and lack of integrated platforms.
  • Existing microfluidic systems often lack a generic, user-friendly interface for diverse laboratory operations.

Purpose of the Study:

  • To develop a novel microfluidic platform that replicates multiwell plate functionality in a 2D droplet array.
  • To enable seamless encapsulation, monitoring, content manipulation, and selective extraction of nanoliter droplets for biological assays.

Main Methods:

  • A platform was engineered using surface-energy anchors to create a 2D array of nanoliter droplets.
  • Bacterial cells were encapsulated in liquid or hydrogel droplets for culture and analysis.
  • Selective droplet extraction was achieved via laser-induced liquid-to-gel transition.

Main Results:

  • The platform successfully demonstrated encapsulation, time-resolved monitoring, and content variation of thousands of individual bacterial cells.
  • Hydrogel droplets facilitated antibiotic gradient application for a single-experiment antibiogram.
  • Precise quantification was achieved using standard and digital enumeration methods.

Conclusions:

  • This integrated droplet microfluidic platform enhances accessibility and simplifies complex biological experiments.
  • The system provides a robust environment for relating genotype and phenotype measurements, particularly for bacterial studies.
  • The platform's versatility supports advanced applications like antibiotic susceptibility testing and genetic analysis.