Multiplexed and spatiotemporal measurements of glutamate secreted by neurons and bacteria
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1Department of Chemistry, Seoul National University, Seoul, 08826, Korea. inseong@snu.ac.kr tdchung@snu.ac.kr.
Chemical Communications (Cambridge, England)
|October 11, 2016
Abstract:
By applying an in vivo biotinylation platform, glutamate-sensing protein can be easily immobilized on streptavidin-functionalized magnetic microbeads, which expands the detection modality for the spatiotemporal measurements of glutamate secreted by adherent neuronal cells and suspension microbial cells using fluorescence microscopy and microplate photometers.
