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Updated: Mar 13, 2026
Reverse Transcription Loop-Mediated Isothermal Amplification RT-LAMP Assay for the Specific and Rapid Detection of Tilapia Lake Virus
Published on: May 18, 2020
Simple and rapid method for routine screening of bovine leukemia virus by loop-mediated isothermal amplification
Yuka Okuwa1, Michiko Miyamato-Hayashi, Takaichi Tanaka
1Nanbu Livestock Hygiene Service Center, 324-2 Bo, Saida-machi, Kanazawa, Ishikawa 920-3101, Japan.
Abstract:
Various techniques for screening and detection of bovine leukemia virus (BLV) were compared to ascertain a rapid and simple technique for routine examination. The performance of real-time PCR, nested PCR and loop-mediated isothermal amplification (LAMP) assays was compared using DNA extracted from whole blood instead of white blood cells (WBCs) of 23 cattle. Real-time PCR, LAMP and nested PCR detected 18, 16 and 11 BLV-positive cattle, respectively. These results suggest that LAMP using DNA from whole blood could enable rapid examination, as isolation of WBCs and electrophoresis is time-consuming and could be useful as a simple and rapid method for routine screening of BLV.
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