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Reference methods--with special reference to cholesterol.

S Stender1

  • 1Department of Clinical Chemistry, Hvidovre Hospital, University of Copenhagen, Denmark.

Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum
|January 1, 1989
PubMed
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A Danish hierarchy of methods for total cholesterol determination ensures accuracy by linking laboratory results to reference methods. Proficiency studies confirm the reliability of these methods for serum cholesterol analysis.

Area of Science:

  • Clinical Chemistry
  • Analytical Chemistry
  • Biochemistry

Background:

  • A hierarchy of methods was established in Denmark in 1982 for accuracy assurance of total cholesterol determination in serum.
  • This system allows laboratories to relate their measurements to reference methods adjusted to definitive methodology.

Purpose of the Study:

  • To establish a system for accuracy assurance in total cholesterol determination.
  • To validate the hierarchy of methods through proficiency studies.
  • To highlight the importance of understanding variation in serum cholesterol measurements.

Main Methods:

  • Establishment of a hierarchy of methods for total cholesterol determination.
  • Conducting proficiency studies in 1985 and 1988 using frozen human serum.

Related Experiment Videos

  • Analysis of interlaboratory coefficients of variation.

    • Main Results:

    • Proficiency studies yielded consensus values for total cholesterol nearly identical to reference methods.
    • Interlaboratory coefficients of variation were consistently low at 4-5%.
    • The established hierarchy effectively ensured accuracy in serum cholesterol measurements.

    Conclusions:

    • The Danish hierarchy of methods provides a reliable framework for accurate total cholesterol determination.
    • Understanding preanalytical, analytical, and biological variations is crucial for interpreting serum cholesterol concentrations.
    • The system facilitates consistent and comparable results across different laboratories.