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Reusable Glucose Sensor Based on Enzyme Immobilized Egg-shell Membrane.

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This study presents a novel glucose biosensor using an egg-shell membrane for immobilizing glucose oxidase. This reusable sensor offers accurate glucose estimation in biological samples with a long shelf-life.

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Area of Science:

  • Biomedical Engineering
  • Biosensor Technology
  • Enzyme Immobilization

Background:

  • Glucose oxidase is crucial for glucose detection.
  • Immobilization of enzymes enhances sensor stability and reusability.
  • Egg-shell membrane offers a sustainable and cost-effective matrix.

Purpose of the Study:

  • To develop a reusable and stable glucose biosensor.
  • To utilize egg-shell membrane for enzyme immobilization.
  • To evaluate the sensor's performance for glucose estimation in biological samples.

Main Methods:

  • Immobilization of glucose oxidase onto egg-shell membrane.
  • Fabrication of a biosensor for glucose detection.
  • Testing sensor performance across a wide glucose concentration range.
  • Assessing sensor reusability and shelf-life.

Main Results:

  • The biosensor demonstrated a wide detection range (1-1000 mM).
  • Fast response times were observed (70s for high, 120s for low concentrations).
  • High sensitivity, a low limit of detection (4.761 mM), and 150 reuse cycles were achieved.
  • A shelf-life exceeding 6 weeks was confirmed.
  • Successful glucose estimation in human blood samples was demonstrated.

Conclusions:

  • Egg-shell membrane is an effective matrix for glucose oxidase immobilization.
  • The developed biosensor is simple, reusable, stable, and suitable for biological samples.
  • This approach offers a promising method for glucose monitoring.