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Updated: Mar 13, 2026

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
Published on: April 4, 2018
Nicotinamide-N-Methyltransferase gene rs694539 variant and migraine risk
Ali Sazci1, Gensay Sazci2, Bilgen Sazci2
1Department of Medical Biology and Genetics, Faculty of Medicine, University of Kocaeli, Kocaeli, 41380, Turkey. alisazci@gmail.com.
Background:
Migraine is a common neurovascular disorder affecting 10 to 20 % of the world population usually subdivided into migraine with auro (MA) and migraine without auro (MO). Homocysteine is involved in the pathophysiology of a number of neurological disorders. Elevated levels of homocysteine in the plasma is produced by the MTHFR gene rs 1801133 and rs 1801131 variants as well as the NNMT gene rs 694539 variant.
Methods:
With the polymerase chain reaction-restriction fragment length polymorphism method developed recently in our laboratory, we were able to show an association between the NNMT gene rs694539 variant and migraine for the first time.
Results:
Here we report the association of the Nicotinamide-N-methyltransferase gene (NNMT) rs694539 variant with migraine in a case-control study of 433 patients with migraine and 229 healthy controls (χ2 = 6.076, P = 0.048). After stratification, we were able only to show an association between the NNMT gene rs694539 variant and female patients with migraine on the genotype and allelic levels. However there was no association in male patients with migraine (χ2 = 1.054, P = 0.590).
Conclusions:
Consequently our results clearly indicate that the NNMT gene rs694539 variant is a genetic risk factor for migraine.
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