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Breakthroughs in Equine Embryo Cryopreservation.

Edward L Squires1

  • 1Department of Veterinary Science, Maxwell H. Gluck Equine Research Center, University of Kentucky, 1500 South limestone Ave, Lexington, KY 40546, USA.

The Veterinary Clinics of North America. Equine Practice
|October 12, 2016
PubMed
Summary

Equine embryo cryopreservation is challenging due to embryo size limitations and difficulties in obtaining sufficient embryos. However, small equine embryos show promising 50-70% pregnancy rates after freezing and thawing.

Keywords:
EmbryoEquineSlow coolVitrification

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Area of Science:

  • Veterinary Science
  • Reproductive Biology
  • Embryology

Background:

  • Equine embryo transfer typically involves fresh or cooled embryos within 24 hours.
  • Cryopreservation of equine embryos offers significant advantages but is rarely utilized.
  • Current limitations hinder widespread adoption of equine embryo freezing techniques.

Purpose of the Study:

  • To investigate the viability and success rates of cryopreserved equine embryos.
  • To identify key factors influencing successful equine embryo cryopreservation and transfer.

Main Methods:

  • Collection of equine embryos from donor mares.
  • Cryopreservation of small equine embryos (<300 μm) using slow cooling or vitrification.
  • Transfer of cryopreserved embryos to recipient mares.

Main Results:

  • Pregnancy rates for small equine embryos after freezing/thawing range from 50% to 70%.
  • Embryo size is a critical factor for successful cryopreservation and subsequent pregnancy rates.
  • Challenges remain in superovulation protocols for obtaining adequate numbers of embryos for freezing.

Conclusions:

  • Cryopreservation of small equine embryos is a viable option, achieving moderate to good pregnancy rates.
  • Further research is needed to improve superovulation techniques and increase embryo availability for freezing.
  • Successful equine embryo cryopreservation holds potential for wider application in assisted reproductive technologies.