Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Substance P: binding to lipids in the brain.

F Lembeck, N Mayer, G Schindler

    Naunyn-Schmiedeberg'S Archives of Pharmacology
    |May 31, 1978
    PubMed
    Summary

    Substance P (SP) is stored in the brain bound to phosphatidylserine, a lipid. This binding is pH-dependent, suggesting a novel mechanism for neuropeptide storage and release.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Role of Spin-Orbit Coupling in High-Order Harmonic Generation Revealed by Supercycle Rydberg Trajectories.

    Physical review letters·2022
    Same author

    Precision Constraints for Three-Flavor Neutrino Oscillations from the Full MINOS+ and MINOS Dataset.

    Physical review letters·2020
    Same author

    Improved Constraints on Sterile Neutrino Mixing from Disappearance Searches in the MINOS, MINOS+, Daya Bay, and Bugey-3 Experiments.

    Physical review letters·2020
    Same author

    The microstructure of laminin-111 compensates for dystroglycan loss in mammary epithelial cells in downstream expression of milk proteins.

    Biomaterials·2019
    Same author

    Search for Sterile Neutrinos in MINOS and MINOS+ Using a Two-Detector Fit.

    Physical review letters·2019
    Same author

    Search for Sterile Neutrinos Mixing with Muon Neutrinos in MINOS.

    Physical review letters·2016

    Area of Science:

    • Neuroscience
    • Biochemistry

    Background:

    • Substance P (SP) is a neuropeptide involved in various physiological processes.
    • The storage and release mechanisms of SP in the brain are not fully understood.

    Purpose of the Study:

    • To investigate the extraction and binding of SP from brain homogenates.
    • To identify the specific brain lipids involved in SP binding.
    • To elucidate the role of pH in SP-lipid interactions.

    Main Methods:

    • Extraction of SP from brain homogenates using a chloroform-methanol method to isolate total lipid extract (TLE).
    • pH-dependent transfer of SP between aqueous and organic phases.
    • Recombination of SP with TLE and assessment of binding capacity.
    • Identification of phosphatidylserine as the key lipid interacting with SP.

    Main Results:

    • SP was efficiently extracted with TLE and could be transferred to aqueous solutions at low pH (2.0-3.0).
    • At higher pH (5.5), SP transferred to an organic phase and bound to TLE with high capacity.
    • Phosphatidylserine was identified as the specific lipid within TLE that binds and releases SP in a pH-dependent manner.

    Conclusions:

    • SP appears to be stored in the brain bound to phosphatidylserine.
    • This SP-phosphatidylserine complex is likely a storage form of SP.
    • The pH-dependent binding suggests a novel storage and potential release mechanism, possibly involving phospholipids as receptors.

    Related Experiment Videos