Related Experiment Video

Updated: Mar 13, 2026

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

8.0K

Chitosan-based nanoparticles for survivin targeted siRNA delivery in breast tumor therapy and preventing its

Ping Sun1, Wei Huang1, Mingji Jin1

  • 1State Key Laboratory of Bioactive Substance and Function of Natural Medicines; Department of Pharmaceutics, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China.

International Journal of Nanomedicine
|October 13, 2016
PubMed

Insights

Poly(ethylene glycol)-modified chitosan (PEG-CS) nanoparticles effectively deliver small interfering RNA (siRNA) to cancer cells. This novel vector enhances therapeutic delivery, significantly inhibiting tumor growth and metastasis in preclinical models.

Area of Science:

  • Biotechnology
  • Nanomedicine
  • Cancer Therapy

Background:

  • Small interfering RNA (siRNA) holds therapeutic potential for cancer but faces challenges in delivery due to degradation.
  • Effective and safe vectors are crucial for successful siRNA-based cancer treatments.

Purpose of the Study:

  • To synthesize and evaluate poly(ethylene glycol)-modified chitosan (PEG-CS) as a safe and efficient vector for siRNA delivery in cancer therapy.
  • To assess the stability, cellular uptake, and antitumor efficacy of PEG-CS/siRNA nanoparticles.

Main Methods:

  • Synthesis of PEG-CS nanoparticles for encapsulating siRNA.
  • Characterization of nanoparticle stability and particle size.
  • In vitro evaluation of cellular uptake and antitumor activity in 4T1 cancer cells.
  • In vivo assessment of tumor growth inhibition in a 4T1 xenograft mouse model.

Main Results:

  • PEG-CS nanoparticles demonstrated superior structural stability and enhanced cellular uptake of siRNA compared to unmodified chitosan nanoparticles.
  • In vitro studies showed significant inhibition of 4T1 tumor cell growth with PEG-CS/siRNA.
  • In vivo experiments revealed that PEG-CS/siRNA nanoparticles effectively reduced xenograft tumor growth and metastasis.

Conclusions:

  • PEG-CS serves as a safe and efficient vector for siRNA delivery.
  • PEG-CS/siRNA nanoparticles show significant potential for reducing tumor growth and preventing metastasis in cancer therapy.
Keywords:
PEGylated chitosanbreast anti-tumor therapynon-viral vectorsiRNA delivery

More Related Videos

Polymalic Acid-based Nano Biopolymers for Targeting of Multiple Tumor Markers: An Opportunity for Personalized Medicine?14:20

Polymalic Acid-based Nano Biopolymers for Targeting of Multiple Tumor Markers: An Opportunity for Personalized Medicine?

Published on: June 13, 2014

17.2K
Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases09:48

Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases

Published on: August 23, 2024

832

Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.9K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
9.1K
Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
79