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Published on: December 15, 2011
NMR-based metabolic characterization of chicken tissues and biofluids: a model for avian research
Caroline Ivanne Le Roy1, Luke John Mappley2, Roberto Marcello La Ragione3
1Department of Food and Nutritional Sciences, University of Reading, Whiteknights, Reading, RG6 6AP UK.
Introduction:
Poultry is one of the most consumed meat in the world and its related industry is always looking for ways to improve animal welfare and productivity. It is therefore essential to understand the metabolic response of the chicken to new feed formulas, various supplements, infections and treatments.
Objectives:
As a basis for future research investigating the impact of diet and infections on chicken's metabolism, we established a high-resolution proton nuclear magnetic resonance (NMR)-based metabolic atlas of the healthy chicken (Gallus gallus).
Methods:
Metabolic extractions were performed prior to 1H-NMR and 2D NMR spectra acquisition on twelve biological matrices: liver, kidney, spleen, plasma, egg yolk and white, colon, caecum, faecal water, ileum, pectoral muscle and brain of 6 chickens. Metabolic profiles were then exhaustively characterized.
Results:
Nearly 80 metabolites were identified. A cross-comparison of these matrices was performed to determine metabolic variations between and within each section and highlighted that only eight core metabolites were systematically found in every matrice.
Conclusion:
This work constitutes a database for future NMR-based metabolomic investigations in relation to avian production and health.
