Related Experiment Video
Updated: May 18, 2026
07:03
Assessing Stomatal Response to Live Bacterial Cells using Whole Leaf Imaging
Published on: October 2, 2010
19.1K
A Rapid and Simple Method for Microscopy-Based Stomata Analyses
Jochen F Eisele1, Florian Fäßler1, Patrick F Bürgel1
1Department of Plant Physiology, Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany.
Plos One
|October 13, 2016
Summary
This study introduces a new rhodamine 6G staining method for Arabidopsis thaliana leaves, simplifying stomatal aperture measurements. This technique minimizes leaf manipulation and speeds up analysis for plant research.
Area of Science:
- Plant Physiology
- Plant Cell Biology
Background:
- Stomatal pore status is assessed via leaf transpiration or direct stomatal aperture measurement.
- Direct measurement often requires invasive leaf manipulation due to poor visualization of cell boundaries in intact leaves.
Purpose of the Study:
- To develop a less invasive and faster protocol for consistent stomatal aperture measurements.
- To enable sequential measurements on the same leaf and facilitate high-throughput analysis.
Main Methods:
- Utilized rhodamine 6G staining for enhanced stomatal visualization in Arabidopsis thaliana.
- Developed a rapid-fixation protocol for epidermal peels to streamline data analysis.
- Applied the method to analyze stomatal responses to stimuli and for long-term monitoring.
Main Results:
- Rhodamine 6G staining significantly simplifies stomatal aperture measurement.
- The protocol allows for minimal leaf manipulation and sequential measurements on the same leaf.
- High-throughput analysis of stomatal responses is achievable with the new method.
Conclusions:
- The rhodamine 6G staining protocol offers a consistent, efficient, and minimally invasive approach for stomatal aperture analysis.
- This method facilitates the study of stomatal dynamics in response to various stimuli and supports parallel analysis of multiple plant lines.
