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Updated: Mar 13, 2026
Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain
Published on: March 13, 2020
Quantitative Analysis of 10 Mycotoxins in Wheat Flour by Ultrahigh Performance Liquid Chromatography-Tandem Mass
Qingxin Zhou1, Fenghua Li1,2, Leilei Chen1
1Inst. of Agro-Food Science & Technology, Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Deep Processing Technology of Agricultural Product, 250100, Jinan, People's Republic of China.
Abstract:
A simple and sensitive analytical method for quantitative analysis of 10 mycotoxins was developed and validated by a combination of modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) procedure with ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Sample preparation involved QuEChERS with dispersive solid phase extraction for clean-up, and analysis was performed by reversed-phase UHPLC-MS/MS using electrospray negative ionization and multiple reaction monitoring. Under optimized conditions, the calibration curves displayed good linear relationships with all coefficients of determinations (r2 ) higher than 0.998. The limits of quantification for all target mycotoxins were lower than 7 μg/kg. Trueness and precision for the analytes were 70% to 116% average recoveries and 2% to 13% relative standard deviations (RSDs). The validated method was used to analyze 46 wheat flour samples for the targeted mycotoxins. The method can be used as a rapid and robust tool for screening mycotoxin in cereal products.
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