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Sequence variation of the P gene among mumps virus strains
A Yamada1, K Takeuchi, K Tanabayashi
1Department of Measles Virus, National Institute of Health, Tokyo, Japan.
Abstract:
We have determined nucleotide sequences of a 183-nucleotide long region of the P gene of 10 mumps virus strains after gene amplification mediated by DNA polymerase catalyzed chain reaction (PCR) and have compared them with those of two strains which had been reported earlier (K. Takeuchi et al., J. Gen. Virol., 69, 2043-2049 (1988]. It was shown that mutation is generally noncumulative, i.e., most nucleotide substitutions in earlier strains do not appear in later strains. Viruses of different lineages appeared to cocirculate, but the comparison of American and Japanese strains suggested that those isolated in one country are more closely related to each other than to those isolated in the other country.
Insights
Mumps virus P gene sequencing revealed noncumulative mutations and cocirculating lineages. Analysis of mumps virus strains suggests geographic isolation influences genetic relatedness.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Mumps virus is a significant human pathogen.
- Understanding mumps virus genetic diversity is crucial for epidemiology and vaccine development.
- Previous studies have provided limited sequence data for the mumps virus P gene.
Purpose of the Study:
- To determine the nucleotide sequences of a 183-nucleotide region of the P gene from 10 mumps virus strains.
- To compare these sequences with previously reported strains to understand genetic variation.
- To investigate the evolutionary patterns and geographic relatedness of mumps virus isolates.
Main Methods:
- Nucleotide sequencing of the P gene region.
- Gene amplification using the polymerase chain reaction (PCR).
- Comparative sequence analysis of mumps virus strains.
Main Results:
- Identified nucleotide sequences for a 183-nucleotide region of the mumps virus P gene in 10 strains.
- Observed that mutations are generally noncumulative, with substitutions in earlier strains not consistently appearing in later ones.
- Found evidence of cocirculating mumps virus lineages.
- American and Japanese mumps virus strains showed closer genetic relatedness within their respective countries compared to strains from the other country.
Conclusions:
- Mumps virus P gene evolution is characterized by noncumulative mutations.
- Geographic isolation appears to play a role in the genetic relatedness of mumps virus strains.
- The findings contribute to understanding mumps virus population dynamics and evolution.