Related Experiment Video

Updated: Mar 13, 2026

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes

Published on: February 8, 2016

The expression of cyclic adenosine monophosphate responsive element modulator in rat sertoli cells following seminal

Muslim Akmal¹, Tongku Nizwan Siregar², Sri Wahyuni³

¹Laboratory of Histology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia.

Veterinary World

|October 14, 2016

Abstract

Aim:

This study aims to determine the effect of seminal vesicle extract on cyclic adenosine monophosphate responsive element modulator (CREM) expression in rat Sertoli cells.

Materials And Methods:

This study examined the expression of CREM on 20 male rats (Rattus norvegicus) at 4 months of age, weighing 250-300 g. The rats were divided into four groups: K0, KP1, KP2, and KP3. K0 group was injected with 0.2 ml normal saline; KP1 was injected with 25 mg cloprostenol (Prostavet C, Virbac S. A); KP2 and KP3 were injected with 0.2 and 0.4 ml seminal vesicle extract, respectively. The treatments were conducted 5 times within 12-day interval. At the end of the study, the rats were euthanized by cervical dislocation; then, the testicles were necropsied and processed for histology observation using immunohistochemistry staining.

Results:

CREM expression in rat Sertoli cells was not altered by the administration of either 0.2 or 0.4 ml seminal vesicle extract.

Conclusion:

The administration of seminal vesicle extract is unable to increase CREM expression in rat Sertoli cells.

Keywords:

Sertoli cells cyclic adenosine monophosphate responsive element modulator seminal vesicle extract spermatogenesis

More Related Videos

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Published on: December 28, 2021

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

Related Concept Videos

Calmodulin-dependent Signaling

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...

Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...

Testosterone: Functions and Regulation

Testosterone: Functions and Regulation

The intricate hormonal interplay essential for male reproductive health begins with the release of gonadotropin-releasing hormone (GnRH) by the hypothalamus. This hormone prompts the pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). LH targets the Leydig cells in the testes, stimulating them to produce and release testosterone. In concert with testosterone, FSH acts on the Sertoli cells within the seminiferous tubules to facilitate the release of...

Articles linked to this work by shared authors, journal, and citation graph.

Molecular detection and VP1-based phylogenetic characterization of Foot-and-mouth disease virus serotype O circulating in cattle in Aceh, Indonesia.

Veterinary world·2026

Fermented foods and probiotic claims: A critical review of scientific validation and regulatory standards.

Nutrition research (New York, N.Y.)·2026

In Vitro Ruminal Fermentation and Methane Production of Fresh and Ensiled TMR Containing Rice Bran Produced in Japan and Indonesia.

Animal science journal = Nihon chikusan Gakkaiho·2026

Transcriptome Analysis Identifies Signaling Pathways Related to Performance, Meat Quality, and Organ Development in Multienzyme-Supplemented Kampung Unggul Balitbangtan (KUB) Chickens.

Veterinary medicine international·2026

Noninvasive Assessment of Testosterone Levels and Male Sexual Behavior in Sambar Deer (Rusa unicolor) as a Critical Step Toward Conservation in Captivity.

Veterinary medicine international·2026

Pituitary extract supplementation enhances follicular survival and gonadotropin receptor expression in vitrified ovarian tissue of Aceh cattle, Indonesia.

Veterinary world·2025

See all related articles