Methods for Studying the Germline of the Human Parasite Schistosoma mansoni

Schistosomes are flatworm parasites that claim the lives of more than 200,000 people in poverty-stricken regions every year. Much of the pathology due to infection is the direct result of injury spurred by the parasite's eggs becoming lodged in host tissues. Thus, asking basic questions about germ cell biology may not only identify novel therapeutic approaches, but could also uncover conserved mechanisms that regulate the germline in diverse metazoa. Here, we detail useful methods for studying the schistosome germline including EdU labeling, whole-mount in situ hybridization, and RNA interference. These methods will hopefully lead to new insights about germline development in the schistosome and facilitate new investigators to begin asking questions about these important and fascinating parasites.