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Author Spotlight: The Production of Recombinant Proteins
Published on: June 30, 2023
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Production of Recombinant CCN Proteins by Brevibacillus choshinensis
Hiroshi Hanagata1, Makoto Mizukami2
1R&D Department, Higeta Shoyu Co. Ltd., 1-5 Hasaki-shinkou, Kamisu, Ibaraki, 314-0407, Japan. h-hanagata@higeta.co.jp.
Methods in Molecular Biology (Clifton, N.J.)
|October 14, 2016
Abstract:
Brevibacillus choshinensis is an excellent host for the production of secretory proteins. This host has also been applied successfully to efficient production of CCN proteins. Described herein are methods of constructing plasmids for CCN protein production (IGFBP-, VWC-, TSP-, and CT-domain) with Brevibacillus as a host, cultivation methods for protein production, and methods of purification for domain proteins using his-tag.
